Hepatitis C is an infectious disease caused by Hepatitis C virus (HCV), which can lead to inflammation and significant damage in the liver. Although it predominantly infects the cells of the liver, it can also affect other parts of the body. During the acute phase following the initial infection of HCV, it is generally asymptomatic and clinically undetectable. About 85 % of the acute infections become chronic and the remaining naturally get cured. In rare cases, acute hepatitis is accompanied by jaundice, malaise, weakness and anorexia. It is estimated that 74 to 86% of individuals with the acute infection develop persistent viremia, which subsequently leads to chronic infection and possibly to cirrhosis or hepatocellular carcinoma.The conventional diagnostic methods include serological testing and liver biopsy. Since HCV cannot be cultured in the clinical laboratory, a sensitive molecular testing is needed to confirm the presence of the virus such as quantitative real-time PCR.